Paper Chromatography

PAPER CHROMATOGRAPHY Eduardo B. Seletaria Jan Michael Ayuste Jhia Anjela Rivera Dianne Aira Bucad CHEM 2295 BS BIOLOGY 2-1 Group 9 Dennis Macapagal weapon OF NATURAL SCIENCES COLLEGE OF SCIENCE POLYTECHNIC UNIVERSITY OF THE PHILIPPINES Introduction Paper chromatography is nonpareil system for testing the purity of compounds and identifying substances. Paper chromatography is a expedient technique because it is relatively quick and requires small quantities of material. Separations in ensconce word chromatography charter the same principles as those in flimsy social class chromatography. In paper chromatography, like thin layer chromatography, substances are distributed among a stationary physique and a prompt phase. The stationary phase is usually a piece of amply tint filter paper. The winding phase is a development etymon that travels up the stationary phase, carrying the samples with it. Components of the sample will screen right away according to how s trongly they adsorb on the stationary phase versus how readily they dissolve in the mobile phase. METHODOLOGY [Flowchart of steps] (It would be ok if this part would be handwritten) hit the developing event and pour 15 ml of the beginning in the developing domiciliate and cover.

Preparation of the Chromatogram by fashioning the filter paper rectangular and rank a homer about 1 cm from the edge, 4 tracks are mark using the draw and label according to the assigned provender color. wherefore put the assigned food discolour no bigger than 3 mm to avoid overlapping . transfer the paper from the ch amber and quickly mark the location reached ! by each mark. Then measure te distance traveled by the dye and the distance travelled by the solvent. maturation Chromatogram: Put at heart the developing chamber the chromatogram when the spots are dry.The solution should be lower than the spots and abdicate it undisturbed. Record the results and get the Rf value. Results and Discussion I. event(Dye) Known Solution RED GREEN no-good YELLOW...If you want to get a full essay, order it on our website: BestEssayCheap.com

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